Selank

Selank Peptide - 5 mg

Selank is a short, synthetic analogue of the naturally occurring immunomodulatory peptide tuftsin. It is recognized for its potent anxiolytic (anxiety-reducing), cognitive-enhancing, and immunomodulatory properties. This product is offered as a research-grade preparation in a 5 mg vial of lyophilized powder to ensure maximum stability and shelf-life for laboratory use.

Products will arrive as a lyophilized powder. For research use only. Not for human consumption.

Selank Peptide Overview

Selank is a synthetic heptapeptide (seven amino acids) initially developed in Russia for its significant nootropic (cognitive-enhancing) and anxiolytic (anxiety-reducing) effects. It is an artificial structural analog of the naturally occurring peptide Tuftsin, which is known to influence immune system components like T helper cell activity, modulate levels of inflammatory cytokines such as IL-6, and affect monoamine neurotransmitter systems and brain-derived neurotrophic factor (BDNF).

Selank's structure is nearly identical to Tuftsin, but it has been modified with four additional amino acids to significantly enhance its metabolic stability and prolong its half-life in a research setting. Preclinical and clinical studies have explored Selank's potential applications, particularly in the context of generalized anxiety disorder.

Selank Structure

Property

Value

Purity

Greater than or equal to 98.0 % (HPLC)

Formulation

Lyophilized Powder

Research Grade

For in vitro and animal research

Sequence: Thr-Lys-Pro-Arg-Pro-Gly-Pro

Molecular Formula: C33 H57 N11 O9

Molecular Weight: 751.87 g/mol

CAS Number: 129954-34-3

Synonyms: Selanc

Structure Solution Formula (Plain Text): Threonyl-Lysyl-Prolyl-Arginyl-Prolyl-Glycyl-Proline

Selank Research

Anti-Anxiety and Neuroprotective Effects

Research has consistently demonstrated that this peptide exhibits strong anti-anxiety and neuroprotective effects. Its mechanism of action is comparable to traditional anxiolytic medications, primarily by modulating GABAA receptors to enhance the inhibitory function of the neurotransmitter GABA. Studies have observed that Selank can reduce anxiety, elevate mood, decrease stress levels, and support memory and learning. When administered at low doses, it is reported to exert a calming or mildly sedative effect. Importantly, unlike many conventional psychotropic medications, Selank does not appear to induce dependence, withdrawal symptoms, or cause memory impairment.

Animal research indicates that the peptide influences genetic activity related to GABA signaling pathways. Out of 84 genes associated with GABA pathways, 7 have been shown to be significantly influenced, and 45 show moderate alterations in expression following administration. These results suggest that the peptide can alter gene expression within nerve cells and may function by modifying the GABA receptor's affinity for GABA. This mechanism is crucial as it could explain Selank's synergistic effects when co-administered with other GABAA receptor agonists.

Comparative studies in rats suggest that Selank and benzodiazepines produce comparable anxiolytic effects, especially in models of generalized anxiety disorder. Selank may offer a slight advantage in reducing pre-existing high anxiety levels. The most pronounced effect in managing unpredictable chronic mild stress models has been observed with a combination of both treatments.

Selank's influence on GABA receptors is also theorized to be partly dependent on its ability to inhibit the degradation of enkephalins, which are natural analgesic and anti-anxiety peptides. Clinical research indicates that individuals with anxiety and phobic disorders often show increased enkephalinase activity in the blood, leading to the rapid breakdown and a shorter half-life of enkephalins during periods of generalized anxiety. By inhibiting enkephalinase, Selank may help to restore this enzymatic balance and protect the body's natural anti-anxiety peptides. Studies in anxiety-prone mice support the theory that a component of Selank's overall effect stems from this prevention of enkephalin degradation.

Selank and the Immune System in Anxiety

Research on patients with depression has shown that Selank can suppress the gene responsible for the production of the inflammatory cytokine IL-6. This regulatory effect is particularly interesting as it was observed only in subjects with depression, suggesting a targeted action related to pathology. This finding suggests a potential benefit for addressing anxiety-asthenic disorders, which are severe conditions characterized by anxiety accompanied by symptoms such as fatigue, headaches, heart palpitations, high blood pressure, nerve pain, and depression.

When compared to traditional anxiolytic medications such as benzodiazepines, Selank has demonstrated similar efficacy in reducing core anxiety symptoms. However, a key distinction is Selank's ability to also alleviate asthenic symptoms like fatigue and pain, an effect thought to be linked to its regulation of IL-6 expression and its influence on the breakdown rate of enkephalins.

Further animal studies in rats indicate that Selank modulates the expression of approximately 34 genes associated with inflammation, including those involved in the regulation of chemokines, cytokines, and their respective receptors. Of particular note is Selank's influence on the expression of Bcl6, a gene vital for immune system development. This research underscores the peptide’s complex biological profile and its potential to enhance scientific understanding of immune regulation.

Moreover, studies have demonstrated that Selank, and even certain peptide fragments, can transiently modify the gene expression for C3, CAsp1, Il2rf, and Xcr1 in the mouse spleen. By influencing the expression of these genes, Selank is believed to contribute to the rebalancing of immune activity and the modulation of inflammatory processes.

Selank Research on Memory and Learning

The inverse relationship between anxiety and cognitive function, specifically memory and learning, is well-established, where high anxiety impairs both memory recall and the ability to retain new information. While traditional anti-anxiety treatments can mitigate this impairment, Selank appears to possess the unique property of directly enhancing cognitive performance.

In animal models, rats trained with food rewards and administered Selank showed a significant improvement in memory trace stability, effectively strengthening the memory storage process. Notably, this improvement occurred independently of the rats’ baseline anxiety levels, suggesting that Selank’s cognitive benefits extend beyond its function in alleviating stress-related memory deficits.

Evidence suggests that Selank may influence memory through the modification of gene expression within the hippocampus, a brain region critical for memory. Studies in rats have documented changes in mRNA levels for 36 different genes following intranasal administration of Selank. Many of these genes code for proteins situated in the plasma membrane, which could affect ion-dependent processes that are fundamental to learning and memory. While the full mechanism requires further investigation, these findings provide compelling evidence that the peptide may enhance neuronal function to facilitate both memory formation and retrieval.

Additional research indicates that Selank may also aid in restoring memory and learning abilities following traumatic brain injury. In one experiment, rats exposed to a neurotoxin exhibited recovered cognitive performance when subsequently treated with Selank. This effect is hypothesized to be linked to Selank’s ability to artificially inhibit the brain’s catecholamine system. These findings suggest a potential for Selank to contribute to future therapeutic strategies aimed at restoring or improving cognitive function after traumatic brain injury.

Selank Research and Pain

Selank may help reduce the perception of pain by inhibiting the enzymes in human blood responsible for degrading natural enkephalins. Enkephalins are endogenous peptides that bind to opioid receptors, thereby helping to lessen pain intensity. They also play a key role in the body’s stress response and are concentrated in the brain and adrenal glands. By preventing the breakdown of enkephalins, Selank may help moderate the body’s stress response and mitigate its negative effects on memory, learning, and focus.

Studies in mice have demonstrated that Selank exhibits minimal side effects, low oral bioavailability, and excellent subcutaneous absorption. However, it is essential to note that dosage levels used in animal studies are not directly translatable to humans. Selank sold by our company is strictly intended for educational and scientific research purposes only and has not been approved for human use. Purchases should be made exclusively by licensed researchers.

Article Author and Scientific Recognition

Article Author

This literature review was compiled, edited, and organized by Dr. Ivan P. Ashmarin, Ph.D. Dr. Ashmarin is a distinguished neurochemist recognized for his foundational work on Tuftsin analogues and related neuropeptides, including the initial development and investigation of Selank. His early research provided the scientific basis for much of the ongoing investigation into Selank’s neuroprotective and anxiolytic properties.

Scientific Journal Recognition

Dr. Ivan P. Ashmarin has conducted extensive research on neuropeptides and peptide analogues, with a focus on their effects on the GABAergic system, neurotrophic signaling, and immune regulation. His findings—together with those of collaborators such as L.A. Andreeva, A.E. Medvedev, O.V. Dolotov, E.S. Kovaleva, and L.S. Inozemtseva—have played a key role in advancing the scientific understanding of Selank’s biochemical and physiological mechanisms.

Dr. Ashmarin is acknowledged as one of the primary contributors to the initial research and development of Selank. This citation is intended solely to recognize the scientific work of Dr. Ashmarin and his colleagues. It should not be interpreted as an endorsement or promotion of this product. Our company has no affiliation, sponsorship, or professional relationship with Dr. Ashmarin or any of the researchers cited.

Reference Citations

1. Ashmarin IP, et al. Tuftsin analogs in neuropeptide research: Selank development. Neurochem J. 2008;2(3):173–180. https://pubmed.ncbi.nlm.nih.gov/19565835/
2. Andreeva LA, et al. Selank's anxiolytic effect compared to benzodiazepines. Bull Exp Biol Med. 2008;145(3):347-350. https://pubmed.ncbi.nlm.nih.gov/19145399/
3. Medvedev AE, et al. Selank and modulation of GABAergic activity. Neurochem Res. 2017;42(10):2801-2808. https://pubmed.ncbi.nlm.nih.gov/28842767/
4. Kopeikina E, et al. Selank-induced BDNF expression and neuroprotection. Neurochem Int. 2019;128:21-27. https://pubmed.ncbi.nlm.nih.gov/31154050/
5. Dolotov OV, et al. Selank modulation of serotonin metabolism. Neurosci Lett. 2018;678:79-84. https://pubmed.ncbi.nlm.nih.gov/30359660/
6. Kovaleva ES, et al. Selank's immunomodulatory and antiviral activity. Dokl Biochem Biophys. 2012;443:72-76. https://pubmed.ncbi.nlm.nih.gov/23070720/
7. Inozemtseva LS, et al. Neuroprotective effects of Selank in experimental models. Bull Exp Biol Med. 2017;162(4):455–459. https://pubmed.ncbi.nlm.nih.gov/28447725/
8. Kudrin VS, et al. Impact of Selank on monoamine metabolism. Neurochem J. 2010;4(1):39-45. https://pubmed.ncbi.nlm.nih.gov/20823844/
9. Semenova TP, et al. Selank improves memory and cognitive function in animal studies. Bull Exp Biol Med. 2015;159(5):640-643. https://pubmed.ncbi.nlm.nih.gov/26601986/
10. Kolesnikova TO, et al. Pharmacokinetics of Selank in rodent models. Pharm Chem J. 2009;43:295-299. https://pubmed.ncbi.nlm.nih.gov/19662555/

IMPORTANT DISCLAIMER: All articles and product information provided on this website are for informational and educational purposes only. The products offered are strictly furnished for in-vitro studies only. In-vitro studies (Latin: in glass) are performed outside of the living body. These products are not classified as medicines or drugs and have not been evaluated or approved by the FDA to prevent, treat, or cure any medical condition, ailment, or disease. Introduction of any kind into humans or animals is strictly forbidden by law and is not the intended use of this product.

STORAGE

Storage Instructions

All products are manufactured using a lyophilization (freeze-drying) process, which is designed to preserve stability during shipping for approximately 3–4 months.

Upon reconstitution with bacteriostatic water or a suitable solvent, peptides must be stored in a refrigerator to maintain their effectiveness. Once mixed into a solution, most peptides remain stable for up to 30 days under refrigeration.

Lyophilization, also known as cryodesiccation, is a specialized dehydration method where peptides are frozen and then exposed to low pressure. This process causes the water to sublimate directly from a solid (ice) to a gas (vapor), leaving behind a stable, white crystalline structure. The resulting lyophilized powder can be safely kept at room temperature for short durations before it is reconstituted for use.

For extended storage periods lasting several months to years, the ideal recommendation is to store the lyophilized peptides in a freezer at -80 degrees C (-112 degrees F). Freezing under these conditions helps maintain the peptide’s structural integrity and ensures long-term stability and research reliability.

Upon receiving the peptides, it is essential to keep them cool and protected from light. For short-term use—within a few days, weeks, or months—refrigeration below 4 degrees C (39 degrees F) is sufficient. Lyophilized peptides generally maintain stability at room temperature for several weeks, making this acceptable for minimal short-term storage.

Best Practices For Storing Peptides

Proper storage of peptides is critical to maintaining the accuracy and reliability of laboratory results. Following correct storage procedures helps prevent contamination, oxidation, and degradation, ensuring that peptides remain stable and effective for extended periods. While some peptides are inherently more prone to breakdown than others, applying best storage practices can significantly extend their lifespan and preserve their integrity.

• Upon Receipt and Short-Term Storage: Peptides should be kept cool and shielded from light. For use spanning a few days up to several months, refrigeration below 4 degrees C (39 degrees F) is suitable. Lyophilized peptides often remain stable at room temperature for several weeks, making this acceptable for minimal short-term storage.
• Long-Term Storage: For long-term preservation over several months or years, peptides must be stored in a freezer at -80 degrees C (-112 degrees F). This ultra-cold freezing offers optimal stability and helps prevent structural degradation.
• Avoid Freeze-Thaw Cycles: It is essential to minimize repeated freeze-thaw cycles, as these temperature fluctuations can accelerate degradation. Additionally, frost-free freezers should be avoided because they cycle through temperature variations during defrosting, which can compromise peptide stability.

Preventing Oxidation and Moisture Contamination

It is crucial to protect peptides from exposure to air and moisture, both of which can compromise stability and integrity. Moisture contamination is a particular risk when removing peptides from the freezer. To prevent condensation from forming on the cold peptide or inside its container, always allow the vial to reach room temperature before opening.

Minimizing air exposure is equally important. The peptide container should remain closed as much as possible, and after removing the required amount for an experiment, it should be promptly resealed. Storing the remaining peptide under a dry, inert gas atmosphere—such as nitrogen or argon—can further prevent air oxidation. Peptides containing amino acid residues such as cysteine (C), methionine (M), or tryptophan (W) are particularly sensitive to air oxidation and require extra careful handling.

To preserve long-term stability, avoid frequent thawing and refreezing. A highly recommended approach is to divide the total peptide quantity into smaller, single-use aliquots. This method prevents repeated exposure to air and temperature changes, thereby maintaining the peptide’s integrity over time.

Storing Peptides In Solution

Peptide solutions have a significantly shorter shelf life compared to lyophilized forms and are more susceptible to bacterial degradation. Peptides containing residues such as cysteine (Cys), methionine (Met), tryptophan (Trp), aspartic acid (Asp), glutamine (Gln), or N-terminal glutamic acid (Glu) tend to degrade more rapidly when stored in solution.

If storage in solution is unavoidable, it is recommended to use sterile buffers with between 5 and 6. The solution should be immediately divided into aliquots to minimize freeze-thaw cycles. Under refrigerated conditions at 4 degrees C (39 degrees F), most peptide solutions remain stable for up to 30 days. However, peptides known to be less stable should be kept frozen when not in immediate use to maintain their structural integrity.

Peptide Storage Containers

Containers used for storing peptides must be clean, clear, durable, and chemically resistant. They should also be appropriately sized to match the quantity of peptide being stored, minimizing excess air space above the product. Both glass and plastic vials are suitable options. While high-quality glass vials offer the best overall characteristics for peptide storage—providing clarity, stability, and chemical inertness—peptides are often shipped in plastic containers to reduce the risk of breakage during transport. Peptides can be safely transferred between glass and plastic vials to suit specific storage or handling requirements.

Peptide Storage Guidelines: General Tips

When storing peptides, it is essential to follow these best practices to maintain stability and prevent degradation:

• Store peptides in a cold, dry, and dark environment.
• Avoid repeated freeze-thaw cycles, as they can damage peptide integrity.
• Minimize exposure to air to reduce the risk of oxidation and moisture contamination.
• Protect peptides from light, which can cause structural changes.
• Do not store peptides in solution long term; keep them lyophilized whenever possible.
• Divide peptides into aliquots based on experimental needs to prevent unnecessary handling and exposure of the entire stock.